Studies on the allergy-like food poisoning associated with putrefaction of marine products. II. Separation of causative substance and some of its chemical characteristics.

All of the four distinct outbreaks of allergy-like food poisoning occurred in Hiroshima in February, 1953, in Shizuoka in July, 1954, both by •gsamma sakuraboshi•h, in Kumamoto in July, 1953, by canned seasoned mackerel and Kanagawa in August, 1954, by cooked frigate mackerel, were investigated by the authors. In all the incriminated materials, the presence of unusually large amount of histamine and other vagus-stimulant, though of unknown structure, was also demonstrated, which has been the subject of the authors' previous report, I(1). The present study was aimed at the isolation of the unidentified toxic substance to be investigated for its chemical properties. The isolation was successfully achieved by means of paper chromatography, and the poison thus isolated has been tentatively designated as •gsaurine•h by the authors.